![]() ![]() The improved iMango-III and Mango-III(A10U) fluoresce ~50% brighter than enhanced green fluorescent protein, making them suitable tags for live cell RNA visualization. The fluorophore is restrained into a planar conformation by the G-quadruplex, a lone, long-range trans Watson-Crick pair (whose A10U mutation increases quantum yield to 0.66), and a pyrimidine perpendicular to the nucleobase planes of those motifs. The structures reveal a globular architecture arising from an unprecedented pseudoknot-like connectivity between a G-quadruplex and an embedded non-canonical duplex. We report crystal structures of TO1-Biotin complexes of Mango-III, a structure-guided mutant Mango-III(A10U), and a functionally reselected mutant iMango-III. Uniquely among related aptamers, Mango-III exhibits biphasic thermal melting, characteristic of molecules with tertiary structure. Among these, the ~30 nucleotide Mango-III is notable because it binds the thiazole orange derivative TO1-Biotin with high affinity and fluoresces brightly (quantum yield 0.55). original sound - Yer a Rizzard Harry.Several turn-on RNA aptamers that activate small-molecule fluorophores have been selected in vitro. TikTok video from Yer a Rizzard Harry "Most Of You Will Have Already Known These But I Might Aswell Share Them For The People That Don’t! □ (Don’t Forget To Like & Follow For Weekly Content) ‼️ #harrypotter #harrypottertiktok #harrypotteredit #harrypottertok #harrypotterstories #harrypotterfan #harrypottermovies #harrypotterpov #harrypottertheory #harrypottertheme #harrypotterprisonerofazkaban #fantasticbeasts #fantasticbeast #fantasticbeastsedit #harrypotterlore #harrypotterlocations #harrypottereasteregg #harrypotter3 #harrypotterfanfic #harrypotterfandom #harrypotterfacts #harrypotterfyp #harrypotterfans #20yearsofmoviemagic #20yearsofharrypotter #marauders #maraudersmap #page394 #thegoldentrio #dracomalfoy #emmawatson #danielradcliffe #rupertgrint #prisonerofazkaban #hogwartsstories #hogwarts #hogwartsismyhome #hogwartslegacy #harrypotterstory #moreharrypotter #avadakedavra #timeturner #fy #fyp #fypシ #fypage #fypシ゚viral #fypdongggggggg #fypp #fyppppppppppppppppppppppp #fyy #fypdong #harrypotterfilm #harrypotterfilms #wb #tomfelton #expelliarmus #wandsup #harrypottertheories #harrypotterreunion #harrypottehistorys #harrypotterworld #harrypotterwizard #harrypotterwands #harrypotterreact #harrypotterquestions #harrypottergroupchat #harrypotterdiy #harrypottercharacters #harrypottercosplay #harrypottercast #harrypottervibes #harrypottermemes ‼️". I (see rule 8) APPLICATION FOR AUTHORISATION (To be submitted in duplicate.). Most Of You Will Have Already Known These But I Might Aswell Share Them For The People That Don’t! □ (Don’t Forget To Like & Follow For Weekly Content) ‼️ #harrypotter #harrypottertiktok #harrypotteredit #harrypottertok #harrypotterstories #harrypotterfan #harrypottermovies #harrypotterpov #harrypottertheory #harrypottertheme #harrypotterprisonerofazkaban #fantasticbeasts #fantasticbeast #fantasticbeastsedit #harrypotterlore #harrypotterlocations #harrypottereasteregg #harrypotter3 #harrypotterfanfic #harrypotterfandom #harrypotterfacts #harrypotterfyp #harrypotterfans #20yearsofmoviemagic #20yearsofharrypotter #marauders #maraudersmap #page394 #thegoldentrio #dracomalfoy #emmawatson #danielradcliffe #rupertgrint #prisonerofazkaban #hogwartsstories #hogwarts #hogwartsismyhome #hogwartslegacy #harrypotterstory #moreharrypotter #avadakedavra #timeturner #fy #fyp #fypシ #fypage #fypシ゚viral #fypdongggggggg #fypp #fyppppppppppppppppppppppp #fyy #fypdong #harrypotterfilm #harrypotterfilms #wb #tomfelton #expelliarmus #wandsup #harrypottertheories #harrypotterreunion #harrypottehistorys #harrypotterworld #harrypotterwizard #harrypotterwands #harrypotterreact #harrypotterquestions #harrypottergroupchat #harrypotterdiy #harrypottercharacters #harrypottercosplay #harrypottercast #harrypottervibes #harrypottermemes ‼️ĩ.3K Likes, 20 Comments. Remaining 70 of the funds would be retained by the IMA/NGO/partner to meet. ![]()
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